產品英文名稱：Activin A,human
產品中文名稱: 人激活素A

Activin A,human產品介紹：
激活素(activins) Activin A是轉化生長因子(TGF)β家族的一種分泌蛋白，可控制胚胎軸發(fā)育為有功能的前腸源性組織，調解多種細胞的生長和分化。多種研究表明，其能誘導胰腺β細胞的分化。尤其與其他因子的聯合作用后能發(fā)揮更顯著的誘導作用。如activin A聯合全反式維甲酸可以更有效地誘導鼠ESC為產胰島素細胞，而activin與BTC共同作用可以轉化新生大鼠導管細胞為產胰島素細胞。卵泡抑素(follistatin)和表皮生長因子相關肽(Cripto)是ac2tivins的天然拮抗劑，有研究表明了胰島分化階段導管上皮細胞里的activins及其抑制因子follistatin和Cripto都有所增加,卵泡抑素抑制activin的同時，能顯著促進胰腺導管細胞新生而減少β細胞的分化，故選擇抑制activin信號途徑可運用于擴增前體細胞，而激活activin有利于β細胞的分化。這些結果表說明,胰腺生長和分化之間的平衡可通過activin信號途徑的精確調節(jié)來實現。同時它還被廣泛用于干細胞研究，用來誘導分化的胚胎干細胞形成內胚層。

Activin A,human 產品特性：
? Activin A,human內毒素含量小于0.1EU /μg，活性經過人源細胞鑒定，純度高
? 生物活性在人源293細胞經過SMAD實驗鑒定The EC50為 0.5–2.0 ng/ml
? Activin A,human產品經過SDS-PAGE 和HPLC雙層鑒定純度大于95%
? 只限于科學研究，非人體使用

Activin A,human 產品英文簡述：
Activin A is a member of the TGFβ superfamily. It is a 26 kDa disulfidelinked homodimer of two βA chains, each containing 116 amino acid residues. Activin A is widely used in stem cell research. It has been used to induce the differentiation of embryonic stem cells to the definitive endoderm. The biological activities of Activin A can be neutralized by diffusible TGF-β antagonists, Follistatin (also available from StemRD). StemRD’s Activin A is made in human 293 cells, which renders human-specific post-translational modifications. We purified this product through a series of chromatography including HPLC to near homogeneity.

? Purity: Greater than 95% by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.5 – 2.0 ng/ml
? For Research Use Only. Not for Use in Humans