英文名稱：Recombinant Mouse Epidermal Growth Factor
中文簡稱：【mEGF】重組小鼠表皮生長因子

Recombinant Mouse Epidermal Growth Factor 【mEGF】重組小鼠表皮生長因子 細(xì)胞因子產(chǎn)品描述：

EGF（Epidermal Growth Factor）中文名稱表皮生長因子，是一類重要的生長因子。EGF是有53個氨基酸分子組成的肽鏈，鏈中含有6個半胱氨酸分子，半胱氨酸分子間形成穩(wěn)定的雙硫鍵，使整條肽鏈成為三個環(huán)聯(lián)部分的活性物質(zhì)。EGF的主要作用是促進(jìn)皮膚細(xì)胞的增殖、分化，加速受傷表皮細(xì)胞的修復(fù)，加速新陳代謝，提高新細(xì)胞在皮膚中所占的比例，它能起到延緩皮膚細(xì)胞衰老，使皮膚滋潤光滑的作用。還有抑制胃酸分泌等特殊的效果，在醫(yī)藥上有很廣的用途。
小鼠表皮生長因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有53個氨基酸（包括3個分子間二硫鍵），分子量為6kDa。該小鼠表皮生長因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---大腸桿菌中重組表達(dá)

Recombinant Mouse Epidermal Growth Factor 【mEGF】重組小鼠表皮生長因子 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的小鼠表皮生長因子(mEGF)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml小鼠表皮生長因子(mEGF)的1x PBS （pH 7.4）溶液中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100ug/ml，后繼實驗過程中可根據(jù)實驗要求進(jìn)一步稀釋。
保存：小鼠表皮生長因子(mEGF)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Mouse Epidermal Growth Factor 【mEGF】重組小鼠表皮生長因子 細(xì)胞因子產(chǎn)品英文描述：
Synonyms 

Urogastrone, URG, EGF.

Introduction 

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description 

Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.
The EGF is purified by proprietary chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH-7.4.

Solubility  （溶解性）

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability   （穩(wěn)定性）

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity   （純度）

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human.

Biological Activity     （生物活性）

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000 units/mg.

Protein content    （蛋白質(zhì)含量）

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 3 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.