產(chǎn)品英文名稱：mTeSR?1
產(chǎn)品中文名稱：iPSC/ESC無血清培養(yǎng)基

STEMCELL iPSC/ESC無血清培養(yǎng)基 化學(xué)成分確定的 mTeSR?1（產(chǎn)品號(hào) #05850）/mTeSR?2 （產(chǎn)品號(hào)#05860）產(chǎn)品簡介：
STEMCELL Technologies 研發(fā)的mTeSR?1（產(chǎn)品號(hào) #05850）和 mTeSR?2（產(chǎn)品號(hào)#05860），作為無飼養(yǎng)層細(xì)胞條件下維持 hPSC 的標(biāo)準(zhǔn)化培養(yǎng)基。它們的成分基于 Ludwig等公布的研究成果，是完全的、成分確定的無血清培養(yǎng)基，其生產(chǎn)得到 WiCell Research Institute 的授權(quán)。mTeSR?1含牛血清白蛋白來源，用于支持長期、無飼養(yǎng)層細(xì)胞條件下培養(yǎng) hPSC 以及在無飼養(yǎng)層細(xì)胞條件下衍生 hiPSC。TeSR?2則是不含動(dòng)物蛋白的配方，同樣支持長期、無飼養(yǎng)層細(xì)胞條件下培養(yǎng) hPSC。兩者均不需要進(jìn)一步添加生長因子。它們旨在維持和擴(kuò)增未分化的hPSC，并與作為基質(zhì)的 BD Matrigel? hESC-qualified Matrix（BD 產(chǎn)品號(hào)#354277）配合使用。STEMCELL Technologies 預(yù)先對每一批次的 BD Matrigel?的質(zhì)量進(jìn)行檢測，確保均一性、可重復(fù)性和可靠性。若要獲得完全不含動(dòng)物蛋白的培養(yǎng)體系，TeSR?2 可與Vitronectin第替代基質(zhì)配合使用。
在 mTeSR?1 和 TeSR?2 中培養(yǎng)的 hPSC：
·? 表型均勻且核型正常
·? 表達(dá)高水平的多能性標(biāo)記如：Oct-3/4、SSEA-3、SSEA-4、TRA-1-60、TRA-1-81
·? 表達(dá)多能性所需的基因：Oct-3/4、nanog
·? 形成包含內(nèi)、中、外胚層譜系衍生物的畸胎瘤
·? 可在體外分化為功能成熟的細(xì)胞類型（如造血細(xì)胞）
·? 從基于飼養(yǎng)層細(xì)胞的培養(yǎng)物轉(zhuǎn)化時(shí)，不需要馴化培養(yǎng)期（即無細(xì)胞低產(chǎn)期）
使用 mTeSR?1 和 TeSR?2 的優(yōu)點(diǎn)包括：
·? 去除不確定的培養(yǎng)基成分和消除與飼養(yǎng)層細(xì)胞和條件培養(yǎng)基有關(guān)的固有可變性，使 hPSC 培養(yǎng)的條件保持一致性
·? 標(biāo)準(zhǔn)化培養(yǎng)方法，從而提高數(shù)據(jù)的可重復(fù)性
·? 省去制備飼養(yǎng)層細(xì)胞或條件培養(yǎng)基的程序，節(jié)省了時(shí)間和勞力
·? 完全的培養(yǎng)基配方，無需添加額外的生長因子或其他添加物

STEMCELL iPSC/ESC無血清培養(yǎng)基 化學(xué)成分確定的 mTeSR?1（產(chǎn)品號(hào) #05850）/mTeSR?2 （產(chǎn)品號(hào)#05860）相關(guān)產(chǎn)品訂購信息：
Product Name ?Description ?Catalog # ?Size ?Price ?Quantity
mTeSR?1 ?Defined, feeder-free maintenance medium for human ESCs and iPSCs ?05850 ?500 mL Kit ?Request pricing
mTeSR?1 ?Defined, feeder-free maintenance medium for hESCs and hiPSCs, 1 L kit ?05857 ?1 L Kit ?Request pricing
mTeSR?1 ?Defined, feeder-free maintenance medium for hESCs and hiPSCs, 10 pack ?05870 ?10 Kits ?Request pricing
mTeSR?1 ?Defined, feeder-free maintenance medium for hESCs and hiPSCs, 25 pack ?05875 ?25 Kits ?Request pricing
STEMCELL iPSC/ESC無血清培養(yǎng)基 化學(xué)成分確定的 mTeSR?1（產(chǎn)品號(hào) #05850）/TeSR?2 （產(chǎn)品號(hào)#05860）英文描述：
mTeSR?1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES cell and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers.
mTeSR?1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR?1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.

STEMCELL iPSC/ESC無血清培養(yǎng)基 化學(xué)成分確定的 mTeSR?1（產(chǎn)品號(hào) #05850）/mTeSR?2 （產(chǎn)品號(hào)#05860）其他相關(guān)產(chǎn)品訂購信息：
Product Name??Description???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? Catalog #
TeSR?2 ?Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells ?05860
AggreWell? ?Simple and Standardized Production of Embryoid Bodies ?27845
mFreSR? ?Defined, Serum-Free Cryopreservation Medium for Human ES cells and iPS cells ?05854
CryoStor? CS10 ?Animal Component-Free, Defined Cryopreservation Medium ?07930
EasySep? hESC-Derived CD34 Positive Selection Kit ?Immunomagnetic Positive Selection Kit ?18167
Mouse IgG (H+L) Antibody, FITC-Conjugated ?Goat Polyclonal Antibody to Mouse IgG (H+L) – FITC Conjugated ?10210
IgM Antibody, FITC-Conjugated ?Goat Polyclonal Antibody to Mouse IgM – FITC Conjugated ?10211
IgM Antibody, APC-Conjugated ?Goat Polyclonal Antibody to Rat IgM – APC Conjugated ?10215
ACCUTASE? ?ACCUTASE? Cell Detachment Solution ?07920
Dispase (1 U/mL) ?Dispase 1 U/mL ?07923
STEMdiff? APEL? Medium ?Serum-Free, Animal Component-Free Medium for Differentiation of Human ES and iPS cells to Multiple Lineage Types ?05210
StemAdhere? Defined Matrix for hPSC ?For maintenance of human ES and iPS cells in combination with mTeSR?1 or TeSR?2 ?07160
Anti-Sox-2 Antibody ?Rabbit Polyclonal Antibody Against Human Sox-2 ?60055
Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480 ?Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-1 (CD15) ?60060
Anti-Mouse SSEA-3 Antibody, Clone MC-631 ?Rat Monoclonal Antibody Against Human, Mouse, Rat SSEA-3 ?60061
Anti-Human SSEA-4 Antibody, Clone MC-813-70 ?Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-4 ?60062
Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R ?Mouse Monoclonal Antibody Against Human, Rhesus, Rabbit TRA-1-60 (Podocalyxin) ?60064
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 ?Mouse Monoclonal Antibody Against Human, Rat, Rhesus TRA-1-81 ?60065
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E ?Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-49 (Tissue Non-Specific Alkaline Phosphatase) ?60066
Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J ?Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-54 (Tissue Non-Specific Alkaline Phosphatase) ?60067
Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 ?Mouse Monoclonal IgM, kappa Isotype Control Antibody ?60069
Gentle Cell Dissociation Reagent ?Enzyme-Free Cell Dissociation Reagent ?07174
STEMdiff? Definitive Endoderm Kit ?Fully Defined Animal Component-Free Medium for the Differentiation of Human ES and iPS Cells to Definitive Endoderm. ?05110
Oct-3/4 Antibody, Clone 40 ?Mouse Monoclonal Antibody to Oct-3/4 ?01550
Vitronectin XF? ?Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions ?07180
STEMdiff? Neural Induction Medium ?Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells