產(chǎn)品英文名稱：Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit
產(chǎn)品中文名稱：Stemcell 19757 EasySep™ 小鼠乳腺干細(xì)胞富集試劑盒

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 產(chǎn)品簡介
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit旨在從新鮮解離的小鼠乳腺組織中分離出乳腺干細(xì)胞。不需要的細(xì)胞被針對非上皮細(xì)胞(CD45, CD31, TER119, BP-1)的生物素化的抗體瞄準(zhǔn)去除。標(biāo)記的細(xì)胞然后被確認(rèn)針對抗生物素和葡聚糖的四聚體的抗體配合物識(shí)別。這些細(xì)胞被綁定到磁性粒子并使用沒有柱的EasySep™磁鐵分離。未受破壞的上皮細(xì)胞富集餾分傾到一個(gè)新的管中，然后用CD24和CD49f抗體標(biāo)記，這樣，CD24+CD49f++ 子種群就可以通過熒光激活細(xì)胞分選分離。

Stemcell 19757 EasySep™ 小鼠乳腺干細(xì)胞富集試劑盒產(chǎn)品組分
Stemcell 19757 EasySep™ 小鼠乳腺干細(xì)胞富集試劑盒有五個(gè)部分
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
• CD24 Antibody, Clone M1/69, PE-Conjugated, 0.5 mL
• CD49f Antibody, Clone GoH3, FITC-Conjugated, 1.0 mL

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 推薦使用
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit is designed for the isolation of mouse mammary stem cells from freshly dissociated mammary tissue by negative selection
Stemcell 19757 EasySep™ 小鼠乳腺干細(xì)胞富集試劑盒所需設(shè)備
• Collagenase/Hyaluronidase Solution (Catalog #07912)
• Gentle Collagenase/Hyaluronidase Solution (Catalog #07919)

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 保存方法
EASYSEP™ MOUSE EPITHELIAL CELL ENRICHMENT COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ BIOTIN SELECTION COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-MOUSE CD24-PE ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-CD49f-FITC ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 實(shí)驗(yàn)資料
FACS Profile Results with EasySep™ Mouse Mammary Stem Cell Enrichment Kit

Freshly dissociated mouse mammary epithelial cells are distributed according to their expression of CD49f and CD24. The mammary repopulating unit (MRU)-rich fraction is found in the CD49f++CD24+ subset. The mammary colony-forming cells (Ma-CFCs) are found in the CD49f+ CD24++ subset. In certain strains of mice (such as C57BI/6J) there can be a small number of CD24++CD49f++ cells (indicated * in the dot plot). Although mammary stem cells have been detected in this fraction, their frequency is much lower than in the MRU fraction.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19757 EasySep™ 小鼠乳腺干細(xì)胞富集試劑盒相關(guān)產(chǎn)品訂購信息
Stemcell  07912  Collagenase/Hyaluronidase  10X Collagenase/Hyaluronidase in DMEM 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  05610  EpiCult™-B Mouse Medium Kit  Medium for Mouse Mammary Epithelial Cell Culture      
For research use only. Not intended for human or animal diagnostic or therapeutic uses.