英文名稱：Recombinant Human Epidermal Growth Factor
中文簡(jiǎn)稱：【EGF】重組人表皮生長(zhǎng)因子

Recombinant Human Epidermal Growth Factor 【EGF】重組人表皮生長(zhǎng)因子 細(xì)胞因子產(chǎn)品描述：

EGF（Epidermal Growth Factor）中文名稱表皮生長(zhǎng)因子，是一類重要的生長(zhǎng)因子。EGF是有53個(gè)氨基酸分子組成的肽鏈，鏈中含有6個(gè)半胱氨酸分子，半胱氨酸分子間形成穩(wěn)定的雙硫鍵，使整條肽鏈成為三個(gè)環(huán)聯(lián)部分的活性物質(zhì)。EGF的主要作用是促進(jìn)皮膚細(xì)胞的增殖、分化，加速受傷表皮細(xì)胞的修復(fù)，加速新陳代謝，提高新細(xì)胞在皮膚中所占的比例，它能起到延緩皮膚細(xì)胞衰老，使皮膚滋潤(rùn)光滑的作用。還有抑制胃酸分泌等特殊的效果，在醫(yī)藥上有很廣的用途。
人表皮生長(zhǎng)因子重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有53個(gè)氨基酸，分子量為6222Da。該人表皮生長(zhǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)

Recombinant Human Epidermal Growth Factor 【EGF】重組人表皮生長(zhǎng)因子 細(xì)胞因子產(chǎn)品實(shí)驗(yàn)須知：
溶解：提供的人表皮生長(zhǎng)因子(EGF)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人表皮生長(zhǎng)因子(EGF)的10mM磷酸鹽緩沖液（pH 7.4）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人表皮生長(zhǎng)因子(EGF)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。

Recombinant Human Epidermal Growth Factor 【EGF】重組人表皮生長(zhǎng)因子 細(xì)胞因子產(chǎn)品英文描述：

Synonyms 

Urogastrone, URG, EGF.

Introduction 

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description 

Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.
The EGF is purified by proprietary chromatographic techniques.

Source  （來(lái)源）

Escherichia Coli.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.

Solubility 

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability  （穩(wěn)定性）

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity  （純度）

Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence  （氨基酸序列）

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.

Biological Activity 

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg.

Protein content 

EGF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.