英文名稱(chēng)：Recombinant Human Vascular Endothelial Growth Factor (121)，Sf9
中文簡(jiǎn)稱(chēng)：【VEGF (121a.a.) Sf9】重組人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá)
Recombinant Human Vascular Endothelial Growth Factor (121)，Sf9 【VEGF (121a.a.) Sf9】重組人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá) 細(xì)胞因子產(chǎn)品描述
血管內(nèi)皮生長(zhǎng)因子（Vascular endothelial growth factor，VEGF）是血管內(nèi)皮細(xì)胞特異性的肝素結(jié)合生長(zhǎng)因子（heparin-binding growth factor），可在體內(nèi)誘導(dǎo)血管新生。VEGF有六種不同亞型，包括VEGF-A，-B，-C，-D，及-E；其分子量從35至44kDa不等，每個(gè)亞型特異性地與三個(gè)“血管內(nèi)皮生長(zhǎng)因子受體”（VEGFR-1，-2，及-3)的特定組合相結(jié)合。VEGF是高度保守的同源二聚體糖蛋白。二條分子量各為24kDa的單鏈以二硫鍵組成二聚體。VEGF分解的單體無(wú)活性,去除N 2糖基對(duì)生物效應(yīng)無(wú)影響，但可能在細(xì)胞分泌中起作用。由于mRNA不同的剪切方式，產(chǎn)生出分別VEGF121、VEGF145、VEGF165、VEGF185、VEGF206等至少5種蛋白形式，其中VEGF121、VEGF145、VEGF165是分泌型可溶性蛋白，能直接作用于血管內(nèi)皮細(xì)胞促進(jìn)血管內(nèi)皮細(xì)胞增殖，增加血管通透性。
人血管內(nèi)皮生長(zhǎng)因子(121)，Sf9表達(dá)重組蛋白是在桿狀病毒-昆蟲(chóng)Sf9細(xì)胞中重組表達(dá)的，是由兩條糖基化多肽鏈構(gòu)成的同源二聚體。包含有121個(gè)氨基酸殘基，分子量為36 kDa。與其它形式的人血管內(nèi)皮生長(zhǎng)因子相比，人血管內(nèi)皮生長(zhǎng)因子(121)循環(huán)更快，與血管硫酸肝素結(jié)合更緊密。該人血管內(nèi)皮生長(zhǎng)因子(121)，Sf9表達(dá)重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---桿狀病毒-昆蟲(chóng)Sf9細(xì)胞中重組表達(dá).
Recombinant Human Vascular Endothelial Growth Factor (121)，Sf9 【VEGF (121a.a.) Sf9】重組人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá) 細(xì)胞因子產(chǎn)品實(shí)驗(yàn)須知:
溶解：提供的人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá)(VEGF (121a.a.) Sf9)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá)(VEGF (121a.a.) Sf9)的溶液（不含添加劑）中凍干產(chǎn)生的。凍干的人血管內(nèi)皮生長(zhǎng)因子具有非常好的水溶性。我們建議加PBS或培養(yǎng)液 （0.1% HSA或BSA載體蛋白）以配制工作儲(chǔ)存液到不低于50ug/ml。
保存：人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá)(VEGF (121a.a.) Sf9)在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Vascular Endothelial Growth Factor (121)，Sf9 【VEGF (121a.a.) Sf9】重組人血管內(nèi)皮生長(zhǎng)因子(121),Sf9表達(dá) 細(xì)胞因子產(chǎn)品英文描述:
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Introduction
Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
Description
Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
The VEGF-121 is purified by proprietary chromatographic techniques.
Source
Sf9, Insect Cells.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a solution containing 50mM acetic acid.
Solubility
The lyophilized VEGF121 should be reconstituted in 50mM acetic acid to a concentration not lower than 50µg/ml.
Stability
Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF121 has been determined to be in the range of 1-4 ng/ml.
Amino Acid Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECV
PTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPR.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
