英文名稱：Recombinant Human beta Nerve Growth Factor，CHO
中文簡稱：【b NGF CHO】重組人β-神經(jīng)生長因子,CHO表達

Recombinant Human beta Nerve Growth Factor，CHO 【b NGF CHO】重組人β-神經(jīng)生長因子,CHO表達產(chǎn)品 細胞因子產(chǎn)品描述：
神經(jīng)生長因子（NGF）是神經(jīng)營養(yǎng)因子中最早被發(fā)現(xiàn)，目前研究最為透徹，具有神經(jīng)元營養(yǎng)和促突起生長雙重生物學功能一種神經(jīng)細胞生長調(diào)節(jié)因子，它對中樞及周圍神經(jīng)元發(fā)育、分化、生長、再生和功能特性表達均具有重要調(diào)控作用。神經(jīng)生長因子包含α、β、γ三個亞單位，活性區(qū)是β亞單位。β-神經(jīng)生長因子由兩個118個氨基酸組成單鏈通過非共價鍵結(jié)合而成二聚體，與人體神經(jīng)生長因子結(jié)構(gòu)具有高度同源性，生物效應(yīng)也無明顯種間特異性。NGF與受體結(jié)合，通過受體介導內(nèi)吞機制產(chǎn)生內(nèi)在化，形成由軸膜包繞、含有NGF、并保持其生物活性小泡，經(jīng)軸突沿微管逆行轉(zhuǎn)運至胞體，經(jīng)酪氨酸蛋白激酶、脂酰肌醇鈣、內(nèi)源性環(huán)腺苷酸等第二信使體系轉(zhuǎn)導，啟動一系列級聯(lián)反應(yīng)，對靶細胞某些結(jié)構(gòu)或功能蛋白基因表達進行調(diào)控而發(fā)揮其生物效應(yīng)。
人β-神經(jīng)生長因子，CHO表達重組蛋白是在CHO中國倉鼠卵巢細胞中重組表達的，是由兩條糖基化多肽鏈組成的同源二聚體，每條多肽鏈包含有120個氨基酸，分子量為16，950Da。該人β-神經(jīng)生長因子，CHO表達重組蛋白最終由標準的色譜技術(shù)純化出。
來源宿主---CHO中國倉鼠卵巢細胞中重組表達

Recombinant Human beta Nerve Growth Factor，CHO 【b NGF CHO】重組人β-神經(jīng)生長因子,CHO表達產(chǎn)品 細胞因子產(chǎn)品使用須知：
溶解：提供的人β-神經(jīng)生長因子,CHO表達(b NGF CHO)產(chǎn)品為經(jīng)0.2um孔徑過濾滅菌的白色凍干粉末；該凍干粉末是從含人β-神經(jīng)生長因子,CHO表達(b NGF CHO)的30mM 乙酸鈉（pH 5.5），1% HSA溶液中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到不低于100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：人β-神經(jīng)生長因子,CHO表達(b NGF CHO)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human beta Nerve Growth Factor，CHO 【b NGF CHO】重組人β-神經(jīng)生長因子,CHO表達產(chǎn)品 細胞因子產(chǎn)品英文描述：
Synonyms 

Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Introduction 

NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Description 

Nerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.
The NGF-b is purified by proprietary chromatographic techniques.

Source （來源）

Chinese Hamster Ovary Cells.

Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a 0.2µm filtered solution in 20mM Tris-HCl pH-8 & 0.15M NaCl.

Solubility  （溶解性）

It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability    （穩(wěn)定性）

Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4C between 2-7 days and for future use below -18C.
For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity   （純度）

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

Was analyzed by Mass spectrometry.

Biological Activity   （生物活性）

The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

Usage 

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.