英文名稱：Recombinant Mouse Tumor Necrosis Factor-alpha
中文簡稱：【mTNF a】重組小鼠腫瘤壞死因子α

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重組小鼠腫瘤壞死因子α 細(xì)胞因子產(chǎn)品描述：

腫瘤壞死因子-α(Tumor Necrosis Factor-alpha，TNF-α)是一種主要由單核-吞噬細(xì)胞產(chǎn)生的單核因子，不僅具有選擇性地殺傷某些腫瘤細(xì)胞，而且有多種免疫調(diào)節(jié)作用。生理條件下具有生物學(xué)活性的人腫瘤壞死因子α（hTNF-α）是一個緊密的三聚體，其活性位點(diǎn)位于兩個相鄰亞基之間的V型結(jié)構(gòu)域，hTNF-α有兩種不同的受體，分別介導(dǎo)不同的生物學(xué)活性，在對hTNF-α結(jié)構(gòu)與功能研究的基礎(chǔ)上，人們對其N末端、C末端和p75受體結(jié)合位點(diǎn)進(jìn)行基因改造。研制了一系列衍生物，以提高h(yuǎn)TNF-α的抗腫瘤活性，降低毒副反應(yīng)。
小鼠腫瘤壞死因子α重組蛋白是在變鉛青鏈霉菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有156個氨基酸，分子量為17301.32Da。該小鼠腫瘤壞死因子α重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---變鉛青鏈霉菌中重組表達(dá)

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重組小鼠腫瘤壞死因子α 細(xì)胞因子產(chǎn)品使用須知：

溶解：提供的小鼠腫瘤壞死因子α(mTNF a)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末中每毫克小鼠腫瘤壞死因子α是從50mM Tris-HCL（pH 8），20mM NaCL溶液中凍干產(chǎn)生的。 我們建議加無菌的18MΩ-cm水以配制工作儲存液到不低于100ug/ml，后繼實(shí)驗過程中可根據(jù)實(shí)驗要求進(jìn)一步稀釋。
保存：小鼠腫瘤壞死因子α(mTNF a)在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重組小鼠腫瘤壞死因子α 細(xì)胞因子產(chǎn)品英文描述：

Synonyms 

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction 

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Description 

Tumor Necrosis Factor-a Mouse Recombinant produced in E. coli is a single, non glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17301.32 Dalton.
The TNF-alpha is purified by standard chromatographic techniques.

Source 

Escherichia Coli.

Physical Appearance  （外觀）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2.

Solubility   （溶解性）

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability    （穩(wěn)定性）

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity     （純度）

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(c) Analysis by SDS-PAGE.

Amino acid sequence    （氨基酸序列）

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Biological Activity     （生物活性）

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.1ng/ml, corresponding to a Specific Activity of 10,000,000 Units/mg.

Protein content       （蛋白質(zhì)含量）

Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.