英文名稱：Recombinant Human Bone Morphogenetic protein-2
中文簡(jiǎn)稱：【BMP 2】重組人骨形態(tài)發(fā)生蛋白-2
Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重組人骨形態(tài)發(fā)生蛋白-2 細(xì)胞因子產(chǎn)品描述：
骨形態(tài)發(fā)生蛋白-2(bone morphogenetic protein-2,BMP-2) 是轉(zhuǎn)化生長(zhǎng)因子β超家族成員之一，具有誘導(dǎo)未分化間充質(zhì)干細(xì)胞向成軟骨細(xì)胞和成骨細(xì)胞定向分化與增殖能力，促進(jìn)成骨細(xì)胞分化成熟，參與骨和軟骨生長(zhǎng)發(fā)育及其重建過(guò)程，進(jìn)而加速骨缺損修復(fù)。BMP-2在體內(nèi)以前體形式合成，經(jīng)蛋白酶切去除信號(hào)肽和前肽，得到由114個(gè)氨基酸殘基組成成熟肽。成熟肽通過(guò)7對(duì)二硫鍵將其保守結(jié)構(gòu)正確折疊。成熟肽同源或異源二聚體才具有生物活性。
人骨形態(tài)發(fā)生蛋白-2重組蛋白是在大腸桿菌中重組表達(dá)的，是由兩條非糖基化多肽鏈組成的同源二聚體，包含有115個(gè)氨基酸，分子量為26018kDa。該人骨形態(tài)發(fā)生蛋白-2重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重組人骨形態(tài)發(fā)生蛋白-2 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的人骨形態(tài)發(fā)生蛋白-2(BMP 2)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人骨形態(tài)發(fā)生蛋白-2(BMP 2)的20mM NaAc（pH 4）溶液中過(guò)濾滅菌并凍干產(chǎn)生的。我們建議加無(wú)菌的20mM AcOH（乙酸）以配制工作儲(chǔ)存液到不低于100ug/ml，后繼實(shí)驗(yàn)過(guò)程 中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人骨形態(tài)發(fā)生蛋白-2(BMP 2)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重組人骨形態(tài)發(fā)生蛋白-2 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
BMP-2, BMP2A.
Introduction
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.
Description
Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2x115 amino acids and having a molecular mass of 26kDa.
The BMP-2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
BMP2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
Solubility （溶解性）
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.
Biological Activity The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 µg/ml.
Protein content （蛋白質(zhì)含量）
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
