產品英文名稱：C-peptide ELISA Kit
產品中文名稱：C-肽ELISA檢測試劑盒

§ 檢測原理：ALPCO C-肽ELISA是一個夾層式免疫檢測試劑盒。96孔微孔板涂有一層C-肽特異性單克隆抗體。將標準物，對照品和樣品一并添加到微型板小孔中與檢測緩沖液混合。然后將微孔板放在搖床上室溫溫育1小時，700－900轉/分鐘。第一次溫育結束，用清洗緩沖液清洗小孔并烘干。添加結合物（配制方法參照說明書），微孔板放在搖床上第二次室溫溫育1小時，700－900轉/分鐘。添加TMB作用底物，放在微孔板搖床上室溫溫育15分鐘，700－900轉/分鐘。第三次溫育結束，添加反應終止液，調節(jié)分光光度計到450nm測定OD值。由于發(fā)光強度與樣品中C-肽含量成比例，因此可以根據OD值確定C-肽含量。

§ 檢測過程：各添加25μl標準物，對照物和樣品→加50µl檢測緩沖液→微孔板搖床室溫溫育1小時，700－900rpm→清洗緩沖液清洗6次→加100µl結合物（配制方法參照說明書）→微孔板搖床室溫溫育1小時，700－900rpm→清洗緩沖液清洗6次→添加100µl TMB作用底物→微孔板搖床室溫溫育15分鐘，700－900rpm→添加100µl反應終止液→分光光度計450nm讀數

ALPCO C-peptide ELISA kit英文簡述：
The ALPCO C-peptide ELISA kit is a FDA registered in vitro diagnostic tool for the quantification of human C-peptide in a clinical setting or research laboratory. Utilizing a dual-monoclonal antibody sandwich ELISA format, bi-level control set, and 96-well microplate comprising removable strips, a single C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components of the C-Peptide ELISA and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

INTENDED USE：The ALPCO C-peptide ELISA is designed for the quantitative determination of C-peptide in human serum and plasma.

PRINCIPLE OF THE ASSAY：The ALPCO C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the Assay Buffer. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The Conjugate is then added, and the microplate is incubated a second time on a microplate shaker at 700-900 rpm, washed, and blotted dry. TMB Substrate is added, and the microplate is incubated a third time at room temperature on a microplate shaker at 700-900 rpm. Once the third incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.